02802nas a2200217   4500008004100000245011600041210006900157260022500226300001300451490000700464520187200471653001402343653001902357653001802376653002702394100001902421700001502440700001702455700001402472856009802486       2007                        eng d00aEffects of selected plant essential oils on the growth and development of mouse preimplantation embryos in vivo0 aEffects of selected plant essential oils on the growth and devel  aAffiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Košice, Slovakia; Affiliation: Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakiac2007///  a97 - 1040 v563 aPlant essential oils (EOs) have been reported to have health benefit properties and their preventive and therapeutic use in animals is expected to increase in the future. We evaluated the influence of five essential oils obtained from plant species which are known to have positive antimicrobial, antioxidative and anti-inflammatory effects - sage EO from Salvia officinalis L. (Lamiaceae), oregano EO from Origanum vulgare L. (Lamiaceae), thyme EO from Thymus vulgaris L. (Lamiaceae), clove EO from Syzygium aromaticum L. (Myrtaceae) and cinnamon EO from Cinnamomum zeylanicum Blume (Lauraceae) on the growth and development of mouse preimplantation embryos in vivo. Essential oils were added to commercial diet at concentrations of 0.25 % for sage EO, thyme EO, clove EO, cinnamon EO and 0.1 % for oregano EO, and fed to ICR female mice for 2 weeks ad libitum. Females were then mated with males of the same strain. Embryos obtained on Day 4 of pregnancy at the blastocyst stage were stained by morphological triple staining (Hoechst, PI, Calcein-AM) and evaluated using fluorescent microscopy. The effects of essential oils were estimated by the viability of embryos, number of nuclei and distribution of embryos according to nucleus number. Cinnamon EO significantly decreased the number of nuclei and the distribution of embryos according to nucleus number was significantly altered. Sage EO negatively influenced the distribution of embryos according to nucleus number. Clove and oregano EOs induced a significantly increased rate of cell death. Only thyme EO had no detectable effects on embryo development. In conclusion, none of the essential oils had any positive effect on embryo development, but some of them reduced the number of cells and increased the incidence of cell death. © 2007 Institute of Physiology, Academy of Sciences of the Czech Republic.10aApoptosis10aEssential oils10aMouse embryos10aPreimplantation embryo1 aDomaracký, M1 aRehák, P1 aJuhás, Š1 aKoppel, J  uhttp://www.scopus.com/scopus/inward/record.url?eid=2-s2.0-33947504242&partnerID=40&rel=R6.5.0