01622nas a2200193   4500008004100000245009500041210006900136260016600205300001400371490000700385520084500392653000901237653001901246653001101265653001501276100001601291700002401307856009701331       2003                        eng d00aValidation of HPLC determination of phenolic acids present in some Lamiaceae family plants0 aValidation of HPLC determination of phenolic acids present in so  aAffiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, Slovak Technical University, Radlinskeho 9, 812 37 Bratislava, Slovakiac2003///  a443 - 4530 v263 aHPLC columns with different length, particle size, and chemical properties of sorbent were tested and compared for the application in the development of the universal HPLC assay for determination of phenolic compounds, which could be present in some medicinal plants from the Lamiaceae family (Melissa officinalis, Rosmarinus officinalis, Salvia officinalis, Thymus serpyllum, and Origanum vulgare). More RP-18 columns have been chosen for the simultaneous separation of all phenolic compounds in the study. The basic chromatographic characteristics were evaluated and the HPLC method using one of the suitable columns was validated for all determined analytes. The recommended separation conditions were applied for phenolic compound monitoring in extracts of plant material. Yields of analytes present in all plant samples were evaluated.10aHPLC10aPhenolic acids10aPlants10aValidation1 aZiaková, A1 aBrandšteterová, E  uhttp://www.scopus.com/scopus/inward/record.url?eid=2-s2.0-0037285983&partnerID=40&rel=R6.5.0